Journal: Scientific Reports

Article Title: Specific chromogenic assay for detection of hippuricase-positive bacteria and its validation in raw chicken meat samples

doi: 10.1038/s41598-025-24981-x

Figure Lengend Snippet: Testing additional hippuricase-positive strain Streptococcus agalactiae ATCC ® 12386 (black bars) and hippuricase-negative Streptococcus pyogenes ATCC ® 19615 (striped, grey bars) with the three oxidizing agents such as hydrogen peroxide/peroxidase (left), enzymatically produced hydrogen peroxide generating system (middle), and using sodium periodate (right). Endpoint absorbance after 15 min. Sterile medium without additives is used as control (empty bars). The reported value represents the average of two measurements under identical conditions.

Article Snippet: Fig. 5 Testing additional hippuricase-positive strain Streptococcus agalactiae ATCC ® 12386 (black bars) and hippuricase-negative Streptococcus pyogenes ATCC ® 19615 (striped, grey bars) with the three oxidizing agents such as hydrogen peroxide/peroxidase (left), enzymatically produced hydrogen peroxide generating system (middle), and using sodium periodate (right).

Techniques: Produced, Sterility, Control

Journal: Communications Biology

Article Title: Growth stage and interspecies interactions shape the cell biology and cell cycle characteristics of human gut bacteria Bacteroides thetaiotaomicron and Roseburia intestinalis

doi: 10.1038/s42003-025-08949-1

Figure Lengend Snippet: A Representative phase contrast and epifluorescence images of B. thetaiotaomicron and R. intestinalis . B Beeswarm plots of the indicated cell morphological features of B. thetaiotaomicron and R. intestinalis cells ( n = 11,987 and 5544 cells, respectively). Darker lines indicate the mean and the standard deviation. C Demographs of Hoechst signal in B. thetaiotaomicron and R. intestinalis cells ( n = 11,987 and 5544 cells, respectively), showing nucleoid dynamics during exponential growth. Cell length is normalized to show the relative cell length in the y-axis. D Schematic line plots indicating the relative and absolute timing of the indicated cell cycle events. E Heatmap scatter plot of the relative nucleoid constriction versus relative cell constriction for B. thetaiotaomicron ( n = 11,987 cells) and R. intestinalis ( n = 5544 cells). ρ = Spearman correlation coefficient.

Article Snippet: B. thetaiotaomicron VPI-5482 (DSM 2079) and R. intestinalis L1-82 (DSM 14610), the type strains of both species, additional B. thetaiotaomicron strains (DSM 24846, DSM 108160, and DSM 108161), and additional Roseburia species ( Roseburia hominis , DSM 16839; Roseburia faecis , DSM 16840; Roseburia inulinivorans , DSM 16841) were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (Leibniz Institute DSMZ GmbH, Germany).

Techniques: Standard Deviation

Journal: Communications Biology

Article Title: Growth stage and interspecies interactions shape the cell biology and cell cycle characteristics of human gut bacteria Bacteroides thetaiotaomicron and Roseburia intestinalis

doi: 10.1038/s42003-025-08949-1

Figure Lengend Snippet: A Representative phase contrast and epifluorescence images of B. thetaiotaomicron and R. intestinalis after pulse labeling with HADA. B Demographs of HADA signal in B. thetaiotaomicron and R. intestinalis cells ( n = 14,370 and 2116 cells, respectively), showing the localized peptidoglycan synthesis patterns during exponential growth. Cell length is normalized to show the relative cell length in the y-axis. C Representative epifluorescence images of R. intestinalis cells with one (top) or two (bottom) PG synthesis zones. The fluorescence intensity profiles of the phase contrast and HADA signal of these cells are shown on the right. D Scatter plot of the relative position of the HADA zone versus cell length. Data points are colored according to their relative cell constriction value.

Article Snippet: B. thetaiotaomicron VPI-5482 (DSM 2079) and R. intestinalis L1-82 (DSM 14610), the type strains of both species, additional B. thetaiotaomicron strains (DSM 24846, DSM 108160, and DSM 108161), and additional Roseburia species ( Roseburia hominis , DSM 16839; Roseburia faecis , DSM 16840; Roseburia inulinivorans , DSM 16841) were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (Leibniz Institute DSMZ GmbH, Germany).

Techniques: Labeling, Fluorescence

Journal: Communications Biology

Article Title: Growth stage and interspecies interactions shape the cell biology and cell cycle characteristics of human gut bacteria Bacteroides thetaiotaomicron and Roseburia intestinalis

doi: 10.1038/s42003-025-08949-1

Figure Lengend Snippet: A Frequency distributions of the indicated cell morphological parameters for B. thetaiotaomicron and R. intestinalis during exponential growth ( n = 11,987 and 5544 cells, respectively) and in stationary phase ( n = 10,450 and 3093 cells, respectively). Mean ± SD and BD (Bhattacharyya distance comparing distributions of exponential and stationary phase) are indicated on the plots. B Frequency distributions of the cell surface-area-to-volume ratio of B. thetaiotaomicron and R. intestinalis cells during exponential growth ( n = 11,987 and 5544 cells, respectively) and in stationary phase ( n = 10,450 and 3093 cells, respectively). Mean ± SD and BD (comparing distributions of exponential and stationary phase) are indicated on the plots. C Bar graphs showing the fraction of constricting B. thetaiotaomicron and R. intestinalis cells during exponential ( n = 11,987 and 5544 cells, respectively) and stationary phase ( n = 10,450 and 3093 cells, respectively). D Frequency distributions of the cell length of constricting cells in B. thetaiotaomicron and R. intestinalis cells during exponential growth ( n = 1960 and 1543 cells, respectively) and in stationary phase ( n = 487 and 460 cells, respectively). Mean ± SD and BD (comparing distributions of exponential and stationary phase) are indicated on the plots.

Article Snippet: B. thetaiotaomicron VPI-5482 (DSM 2079) and R. intestinalis L1-82 (DSM 14610), the type strains of both species, additional B. thetaiotaomicron strains (DSM 24846, DSM 108160, and DSM 108161), and additional Roseburia species ( Roseburia hominis , DSM 16839; Roseburia faecis , DSM 16840; Roseburia inulinivorans , DSM 16841) were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (Leibniz Institute DSMZ GmbH, Germany).

Techniques:

Journal: Communications Biology

Article Title: Growth stage and interspecies interactions shape the cell biology and cell cycle characteristics of human gut bacteria Bacteroides thetaiotaomicron and Roseburia intestinalis

doi: 10.1038/s42003-025-08949-1

Figure Lengend Snippet: A Density contour plots of nucleoid area versus cell area of B. thetaiotaomicron and R. intestinalis cells during exponential growth ( n = 10,090 and 3525 cells, respectively) and in stationary phase ( n = 9308 and 2325 cells, respectively). The contour lines represent the 0.10, 0.25, 0.50, and 0.75 probability envelopes of the data. ρ = Spearman correlation coefficient. B Frequency distributions of the nucleocytoplasmic ratio of B. thetaiotaomicron and R. intestinalis cells during exponential growth ( n = 10,090 and 3525 cells, respectively) and in stationary phase ( n = 9308 and 2325 cells, respectively). Mean ± SD and BD (comparing distributions of exponential and stationary phase) are indicated on the plots. C Demographs of Hoechst signal in B. thetaiotaomicron and R. intestinalis cells ( n = 10,450 and 3093 cells, respectively), showing nucleoid dynamics in stationary phase. Cell length is normalized to show the relative cell length in the y-axis. D Top, representative phase contrast and epifluorescence images of stationary phase B. thetaiotaomicron and R. intestinalis cells. Bottom, phase contrast and fluorescence intensity profiles of the Hoechst and RNASelect signals of these cells.

Article Snippet: B. thetaiotaomicron VPI-5482 (DSM 2079) and R. intestinalis L1-82 (DSM 14610), the type strains of both species, additional B. thetaiotaomicron strains (DSM 24846, DSM 108160, and DSM 108161), and additional Roseburia species ( Roseburia hominis , DSM 16839; Roseburia faecis , DSM 16840; Roseburia inulinivorans , DSM 16841) were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (Leibniz Institute DSMZ GmbH, Germany).

Techniques: Fluorescence

Journal: Communications Biology

Article Title: Growth stage and interspecies interactions shape the cell biology and cell cycle characteristics of human gut bacteria Bacteroides thetaiotaomicron and Roseburia intestinalis

doi: 10.1038/s42003-025-08949-1

Figure Lengend Snippet: A Representative phase contrast images of indicated B. thetaiotaomicron strains during exponential growth. B Frequency distributions of the cell length and cell width for the indicated B. thetaiotaomicron strains during exponential growth ( n = 2150, 5210, 4385, and 3289 cells, respectively) and in stationary phase ( n = 2707, 4319, 1758, and 2588 cells, respectively). Mean ± SD and BD (comparing distributions of exponential and stationary phase) are indicated on the plots. C Demographs of Hoechst signal in the indicated B. thetaiotaomicron strains, showing nucleoid dynamics during exponential growth. Cell length is normalized to show the relative cell length in the y-axis. D Frequency distributions of the nucleocytoplasmic ratio of the indicated B. thetaiotaomicron strains during exponential growth and in stationary phase. Mean ± SD and BD (comparing distributions of exponential and stationary phase) are indicated on the plots.

Article Snippet: B. thetaiotaomicron VPI-5482 (DSM 2079) and R. intestinalis L1-82 (DSM 14610), the type strains of both species, additional B. thetaiotaomicron strains (DSM 24846, DSM 108160, and DSM 108161), and additional Roseburia species ( Roseburia hominis , DSM 16839; Roseburia faecis , DSM 16840; Roseburia inulinivorans , DSM 16841) were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (Leibniz Institute DSMZ GmbH, Germany).

Techniques:

Journal: Communications Biology

Article Title: Growth stage and interspecies interactions shape the cell biology and cell cycle characteristics of human gut bacteria Bacteroides thetaiotaomicron and Roseburia intestinalis

doi: 10.1038/s42003-025-08949-1

Figure Lengend Snippet: A Overview of selected genes involved in growth, cell morphogenesis, and chromosome organization that significantly change in expression between exponential and stationary phase in B. thetaiotaomicron . Color map indicates the log fold changes in gene expression in stationary phase (compared to exponential growth). For genes discussed in the text, commonly used gene names are also included. B Overview of selected genes involved in growth, cell morphogenesis, and chromosome organization that significantly change in expression between exponential and stationary phase in R. intestinalis . Color map indicates the log fold changes in gene expression in stationary phase (compared to exponential growth).

Article Snippet: B. thetaiotaomicron VPI-5482 (DSM 2079) and R. intestinalis L1-82 (DSM 14610), the type strains of both species, additional B. thetaiotaomicron strains (DSM 24846, DSM 108160, and DSM 108161), and additional Roseburia species ( Roseburia hominis , DSM 16839; Roseburia faecis , DSM 16840; Roseburia inulinivorans , DSM 16841) were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (Leibniz Institute DSMZ GmbH, Germany).

Techniques: Expressing, Gene Expression

Journal: Communications Biology

Article Title: Growth stage and interspecies interactions shape the cell biology and cell cycle characteristics of human gut bacteria Bacteroides thetaiotaomicron and Roseburia intestinalis

doi: 10.1038/s42003-025-08949-1

Figure Lengend Snippet: A Representative phase contrast image of a coculture of B. thetaiotaomicron and R. intestinalis during exponential growth. B Frequency distributions of the cell length and cell width of B. thetaiotaomicron and R. intestinalis cells during exponential growth in monoculture ( n = 11,987 and 5544 cells, respectively) and in coculture ( n = 4904 and 242 cells, respectively). Mean ± SD and BD (comparing distributions of exponential and stationary phase) are indicated on the plots. C Frequency distributions of the cell length and cell width of B. thetaiotaomicron and R. intestinalis cells in coculture during exponential growth ( n = 4904 and 242 cells, respectively) and in stationary phase ( n = 4061 and 314 cells, respectively). Mean ± SD and BD (Bhattacharyya distance comparing distributions of exponential and stationary phase) are indicated on the plots. D Frequency distributions of the nucleocytoplasmic ratio of B. thetaiotaomicron cells in coculture during exponential growth ( n = 4904 cells) and in stationary phase ( n = 4061 cells). Mean ± SD and BD (comparing distributions of exponential and stationary phase) are indicated on the plots. E Venn diagram showing the overlap in genes that significantly change in expression between exponential and stationary phase in monoculture and coculture of B. thetaiotaomicron and R. intestinalis .

Article Snippet: B. thetaiotaomicron VPI-5482 (DSM 2079) and R. intestinalis L1-82 (DSM 14610), the type strains of both species, additional B. thetaiotaomicron strains (DSM 24846, DSM 108160, and DSM 108161), and additional Roseburia species ( Roseburia hominis , DSM 16839; Roseburia faecis , DSM 16840; Roseburia inulinivorans , DSM 16841) were obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (Leibniz Institute DSMZ GmbH, Germany).

Techniques: Expressing